An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

WNK regulates Wnt signalling and ?-Catenin levels by interfering with the interaction between ?-Catenin and GID.

Sato A, Shimizu M, Goto T, Masuno H, Kagechika H, Tanaka N, Shibuya H

?-Catenin is an important component of the Wnt signalling pathway. As dysregulation or mutation of this pathway causes many diseases, including cancer, the ?-Catenin level is carefully regulated by the destruction complex in the Wnt signalling pathway. However, the mechanisms underlying the regulation of ?-Catenin ubiquitination and degradation remain unclear. Here, we find that WNK (With No Lysine [K]) kinase ... [more]

Commun Biol Nov. 12, 2020; 3(1);666 [Pubmed: 33184430]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

WNK1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [IDA]chloride channel inhibitor activity [IDA]phosphatase binding [IDA]protein binding [IPI]protein serine/threonine kinase activity [IDA, TAS]

Gene Ontology Cellular Component

MAEA