An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Spt4 facilitates the movement of RNA polymerase II through the +2 nucleosomal barrier.

Uzun Ue, Brown T, Fischl H, Angel A, Mellor J

Spt4 is a transcription elongation factor with homologs in organisms with nucleosomes. Structural and in vitro studies implicate Spt4 in transcription through nucleosomes, and yet the in vivo function of Spt4 is unclear. Here, we assess the precise position of Spt4 during transcription and the consequences of the loss of Spt4 on RNA polymerase II (RNAPII) dynamics and nucleosome positioning in Saccharomyces ... [more]

Cell Rep Sep. 28, 2021; 36(13);109755 [Pubmed: 34592154]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RPB3 SNU56	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gopalakrishnan R (2021)	High	-	BioGRID	3309576
RPB3 SNU56	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.2199	BioGRID	1937362

Curated By

BioGRID

Interaction Summary

RPB3

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II activity [IDA]RNA-directed RNA polymerase activity [IDA]

Gene Ontology Cellular Component

SNU56