BAIT

BIN2

ATSK21, BRASSINOSTEROID-INSENSITIVE 2, DWARF 12, DWF12, F28A21.120, F28A21_120, SHAGGY-LIKE KINASE 21, SK21, UCU1, ULTRACURVATA 1, AT4G18710
Shaggy-related protein kinase eta
Arabidopsis thaliana (Columbia)
PREY

ATMPK8

T10F20.15, AT1G18150
mitogen-activated protein kinase 8
GO Process (5)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Mapping the signaling network of BIN2 kinase using TurboID-mediated biotin labeling and phosphoproteomics.

Kim TW, Park CH, Hsu CC, Kim YW, Ko YW, Zhang Z, Zhu JY, Hsiao YC, Branon T, Kaasik K, Saldivar E, Li K, Pasha A, Provart NJ, Burlingame AL, Xu SL, Ting AY, Wang ZY

Elucidating enzyme-substrate relationships in posttranslational modification (PTM) networks is crucial for understanding signal transduction pathways but is technically difficult because enzyme-substrate interactions tend to be transient. Here, we demonstrate that TurboID-based proximity labeling (TbPL) effectively and specifically captures the substrates of kinases and phosphatases. TbPL-mass spectrometry (TbPL-MS) identified over 400 proximal proteins of Arabidopsis thaliana BRASSINOSTEROID-INSENSITIVE2 (BIN2), a member of ... [more]

Plant Cell Mar. 15, 2023; 35(3);975-993 [Pubmed: 36660928]

Throughput

  • High Throughput

Additional Notes

  • TurboID

Curated By

  • BioGRID