BAIT

CNPY3

CAG4A, ERDA5, PRAT4A, TNRC5, HSPC084
canopy FGF signaling regulator 3
GO Process (2)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens
PREY

NRROS

ELLP3030, GARPL1, LRRC33, UNQ3030, UNQ3030/PRO9833
negative regulator of reactive oxygen species
GO Process (3)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Control of TurboID-dependent biotinylation intensity in proximity ligation screens.

Garloff V, Krueger T, Brakhage A, Rubio I

Proximity biotinylation screens are a widely used strategy for the unbiased identification of interacting or vicinal proteins. The latest generation biotin ligase TurboID has broadened the range of potential applications, as this ligase promotes an intense and faster biotinylation, even in subcellular compartments like the endoplasmic reticulum. On the other hand, the uncontrollable high basal biotinylation rates deny the system's ... [more]

J Proteomics May. 15, 2023; 279();104886 [Pubmed: 36966971]

Throughput

  • High Throughput

Additional Notes

  • TurboID

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NRROS CNPY3
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9988BioGRID
2232937
NRROS CNPY3
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9991BioGRID
3262132
NRROS CNPY3
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9978BioGRID
3061949

Curated By

  • BioGRID