BAIT
CNPY3
CAG4A, ERDA5, PRAT4A, TNRC5, HSPC084
canopy FGF signaling regulator 3
GO Process (2)
GO Function (0)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Homo sapiens
PREY
ATP8A2
ATP, ATPIB, CAMRQ4, IB, ML-1, RP11-141F12.1
ATPase, aminophospholipid transporter, class I, type 8A, member 2
GO Process (1)
GO Function (0)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Control of TurboID-dependent biotinylation intensity in proximity ligation screens.
Proximity biotinylation screens are a widely used strategy for the unbiased identification of interacting or vicinal proteins. The latest generation biotin ligase TurboID has broadened the range of potential applications, as this ligase promotes an intense and faster biotinylation, even in subcellular compartments like the endoplasmic reticulum. On the other hand, the uncontrollable high basal biotinylation rates deny the system's ... [more]
J Proteomics May. 15, 2023; 279();104886 [Pubmed: 36966971]
Throughput
- High Throughput
Additional Notes
- TurboID
Curated By
- BioGRID