BAIT

CNPY3

CAG4A, ERDA5, PRAT4A, TNRC5, HSPC084
canopy FGF signaling regulator 3
GO Process (2)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens
PREY

VCPIP1

DUBA3, VCIP135
valosin containing protein (p97)/p47 complex interacting protein 1
UPS Project
GO Process (3)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Control of TurboID-dependent biotinylation intensity in proximity ligation screens.

Garloff V, Krueger T, Brakhage A, Rubio I

Proximity biotinylation screens are a widely used strategy for the unbiased identification of interacting or vicinal proteins. The latest generation biotin ligase TurboID has broadened the range of potential applications, as this ligase promotes an intense and faster biotinylation, even in subcellular compartments like the endoplasmic reticulum. On the other hand, the uncontrollable high basal biotinylation rates deny the system's ... [more]

J Proteomics May. 15, 2023; 279();104886 [Pubmed: 36966971]

Throughput

  • High Throughput

Additional Notes

  • TurboID

Curated By

  • BioGRID