BAIT

NFKBIA

IKBA, MAD-3, NFKBI
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
GO Process (31)
GO Function (7)
GO Component (5)

Gene Ontology Biological Process

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

AirID, a novel proximity biotinylation enzyme, for analysis of protein-protein interactions.

Kido K, Yamanaka S, Nakano S, Motani K, Shinohara S, Nozawa A, Kosako H, Ito S, Sawasaki T

Proximity biotinylation based on Escherichia coli BirA enzymes such as BioID (BirA*) and TurboID is a key technology for identifying proteins that interact with a target protein in a cell or organism. However, there have been some improvements in the enzymes that are used for that purpose. Here, we demonstrate a novel BirA enzyme, AirID (ancestral BirA for proximity-dependent biotin ... [more]

Elife May. 11, 2020; 9(); [Pubmed: 32391793]

Throughput

  • High Throughput

Additional Notes

  • AirID

Curated By

  • BioGRID