BAIT

FNDC3A

FNDC3, HUGO, bA203I16.1, bA203I16.5, RP11-203I16.5
fibronectin type III domain containing 3A
GO Process (0)
GO Function (1)
GO Component (2)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

FKBP4

FKBP51, FKBP52, FKBP59, HBI, Hsp56, PPIase, p52
FK506 binding protein 4, 59kDa
Alzheimer's Disease Project
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

FAM46C and FNDC3A Are Multiple Myeloma Tumor Suppressors That Act in Concert to Impair Clearing of Protein Aggregates and Autophagy.

Manfrini N, Mancino M, Miluzio A, Oliveto S, Balestra M, Calamita P, Alfieri R, Rossi RL, Sassoe-Pognetto M, Salio C, Cuomo A, Bonaldi T, Manfredi M, Marengo E, Ranzato E, Martinotti S, Cittaro D, Tonon G, Biffo S

Multiple myeloma is a plasma cell neoplasm characterized by the production of unfolded immunoglobulins, which cause endoplasmic reticulum (ER) stress and sensitivity to proteasome inhibition. The genomic landscape of multiple myeloma is characterized by the loss of several genes rarely mutated in other cancers that may underline specific weaknesses of multiple myeloma cells. One of these is FAM46C that is ... [more]

Cancer Res Nov. 01, 2020; 80(21);4693-4706 [Pubmed: 32963011]

Throughput

  • High Throughput

Additional Notes

  • FAM46C/FNDC3A complex
  • False discovery rate (FDR) of all peptide identifications was set to a maximum of 1%.
  • Following double-affinity purification with anti-FLAG (FAM46C) and anti-HA (FNDC3A) antibodies, pulldowns were loaded on gradient gels and seven bands, which were detectable in the FAM46C IP and not in the mock control sample, were identified.
  • HEK293T cell line

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
FNDC3A FKBP4
Cross-Linking-MS (XL-MS)
Cross-Linking-MS (XL-MS)

An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).

High-BioGRID
3746213

Curated By

  • BioGRID