Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

FAM46C and FNDC3A Are Multiple Myeloma Tumor Suppressors That Act in Concert to Impair Clearing of Protein Aggregates and Autophagy.

Manfrini N, Mancino M, Miluzio A, Oliveto S, Balestra M, Calamita P, Alfieri R, Rossi RL, Sassoe-Pognetto M, Salio C, Cuomo A, Bonaldi T, Manfredi M, Marengo E, Ranzato E, Martinotti S, Cittaro D, Tonon G, Biffo S

Multiple myeloma is a plasma cell neoplasm characterized by the production of unfolded immunoglobulins, which cause endoplasmic reticulum (ER) stress and sensitivity to proteasome inhibition. The genomic landscape of multiple myeloma is characterized by the loss of several genes rarely mutated in other cancers that may underline specific weaknesses of multiple myeloma cells. One of these is FAM46C that is ... [more]

Cancer Res Nov. 01, 2020; 80(21);4693-4706 [Pubmed: 32963011]

Throughput

  • High Throughput

Additional Notes

  • FAM46C/FNDC3A complex
  • False discovery rate (FDR) of all peptide identifications was set to a maximum of 1%.
  • Following double-affinity purification with anti-FLAG (FAM46C) and anti-HA (FNDC3A) antibodies, pulldowns were loaded on gradient gels and seven bands, which were detectable in the FAM46C IP and not in the mock control sample, were identified.
  • HEK293T cell line

Curated By

  • BioGRID