BAIT

BPH1

L000003507, YCR032W
Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting
GO Process (3)
GO Function (0)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

MTC1

YJL123C
Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); mtc1 is synthetically lethal with cdc13-1
GO Process (0)
GO Function (0)
GO Component (4)

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

The yeast LYST homolog Bph1 is a Rab5 effector and prevents Atg8 lipidation at endosomes.

Vargas Duarte P, Hardenberg R, Mari M, Walter S, Reggiori F, Froehlich F, Gonzalez Montoro A, Ungermann C

Lysosomes mediate degradation of macromolecules to their precursors for cellular recycling. Additionally, lysosome-related organelles mediate cell type-specific functions. Chediak-Higashi syndrome is an autosomal, recessive disease, in which loss of the protein LYST causes defects in lysosomes and lysosome-related organelles. The molecular function of LYST, however, is largely unknown. Here, we dissected the function of the yeast LYST homolog, Bph1. We ... [more]

J Cell Sci Apr. 15, 2022; 135(8); [Pubmed: 35343566]

Throughput

  • High Throughput

Additional Notes

  • Table S2

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MTC1 BPH1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.0977BioGRID
2444414

Curated By

  • BioGRID