BAIT

SEC61

translocon subunit SEC61, L000001852, YLR378C
Conserved ER protein translocation channel; essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms channel for SRP-dependent protein import; with Sec63 complex allows SRP-independent protein import into ER; involved in posttranslational soluble protein import into the ER, ERAD of soluble substrates, and misfolded soluble protein export from the ER
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

SLO1

S000028475, YER180C-A
Protein interacting with Arl3p; Arl3p is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO
GO Process (1)
GO Function (0)
GO Component (0)

Gene Ontology Biological Process

Saccharomyces cerevisiae (S288c)

Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

A systematic proximity ligation approach to studying protein-substrate specificity identifies the substrate spectrum of the Ssh1 translocon.

Cohen N, Aviram N, Schuldiner M

Many cellular functions are carried out by protein pairs or families, providing robustness alongside functional diversity. For such processes, it remains a challenge to map the degree of specificity versus promiscuity. Protein-protein interactions (PPIs) can be used to inform on these matters as they highlight cellular locals, regulation and, in cases where proteins affect other proteins - substrate range. However, ... [more]

EMBO J Jun. 01, 2023; 42(11);e113385 [Pubmed: 37073826]

Throughput

  • High Throughput

Additional Notes

  • Cel-lctiv (CELlular biotin-Ligation for Capturing Transient Interactions in vivo)
  • Protein that preferentially bound Sec61p compared to Ssh1p.
  • Proximity ligation assay

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SLO1 SEC61
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1263BioGRID
2426670

Curated By

  • BioGRID