BAIT

SEC61

translocon subunit SEC61, L000001852, YLR378C

Conserved ER protein translocation channel; essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms channel for SRP-dependent protein import; with Sec63 complex allows SRP-independent protein import into ER; involved in posttranslational soluble protein import into the ER, ERAD of soluble substrates, and misfolded soluble protein export from the ER

UPS Project

GO Process (6)

GO Function (3)

GO Component (2)

Gene Ontology Biological Process

ER-associated ubiquitin-dependent protein catabolic process [IMP, IPI]

SRP-dependent cotranslational protein targeting to membrane, translocation [IDA]

intracellular protein transmembrane import [IMP]

misfolded protein transport [IMP]

posttranslational protein targeting to membrane, translocation [IDA, IMP]

retrograde protein transport, ER to cytosol [IMP]

Gene Ontology Molecular Function

P-P-bond-hydrolysis-driven protein transmembrane transporter activity [IDA, IMP]
peptide transporter activity [IMP]
signal sequence binding [IDA]

Gene Ontology Cellular Component

Sec61 translocon complex [IDA]

integral component of membrane [ISM]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

RMD6

YEL072W

Protein required for sporulation

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

A systematic proximity ligation approach to studying protein-substrate specificity identifies the substrate spectrum of the Ssh1 translocon.

Cohen N, Aviram N, Schuldiner M

Many cellular functions are carried out by protein pairs or families, providing robustness alongside functional diversity. For such processes, it remains a challenge to map the degree of specificity versus promiscuity. Protein-protein interactions (PPIs) can be used to inform on these matters as they highlight cellular locals, regulation and, in cases where proteins affect other proteins - substrate range. However, ... [more]

EMBO J Jun. 01, 2023; 42(11);e113385 [Pubmed: 37073826]

Throughput

High Throughput

Additional Notes

Cel-lctiv (CELlular biotin-Ligation for Capturing Transient Interactions in vivo)
Protein that bound both Sec61p and Ssh1p with no preference for either protein.
Proximity ligation assay

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RMD6 SEC61	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.1338	BioGRID	2039177

Curated By

BioGRID

Interaction Summary