BAIT

SEC61

translocon subunit SEC61, L000001852, YLR378C
Conserved ER protein translocation channel; essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms channel for SRP-dependent protein import; with Sec63 complex allows SRP-independent protein import into ER; involved in posttranslational soluble protein import into the ER, ERAD of soluble substrates, and misfolded soluble protein export from the ER
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

REB1

GRF2, DNA-binding protein REB1, L000001601, YBR049C
RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; Reb1p bound to DNA acts to block RNA polymerase II readthrough transcription
GO Process (2)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

A systematic proximity ligation approach to studying protein-substrate specificity identifies the substrate spectrum of the Ssh1 translocon.

Cohen N, Aviram N, Schuldiner M

Many cellular functions are carried out by protein pairs or families, providing robustness alongside functional diversity. For such processes, it remains a challenge to map the degree of specificity versus promiscuity. Protein-protein interactions (PPIs) can be used to inform on these matters as they highlight cellular locals, regulation and, in cases where proteins affect other proteins - substrate range. However, ... [more]

EMBO J Jun. 01, 2023; 42(11);e113385 [Pubmed: 37073826]

Throughput

  • High Throughput

Additional Notes

  • Cel-lctiv (CELlular biotin-Ligation for Capturing Transient Interactions in vivo)
  • Protein that bound both Sec61p and Ssh1p with no preference for either protein.
  • Proximity ligation assay

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
REB1 SEC61
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1701BioGRID
1920214

Curated By

  • BioGRID