BAIT
SEC61
translocon subunit SEC61, L000001852, YLR378C
Conserved ER protein translocation channel; essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms channel for SRP-dependent protein import; with Sec63 complex allows SRP-independent protein import into ER; involved in posttranslational soluble protein import into the ER, ERAD of soluble substrates, and misfolded soluble protein export from the ER
GO Process (6)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
- ER-associated ubiquitin-dependent protein catabolic process [IMP, IPI]
- SRP-dependent cotranslational protein targeting to membrane, translocation [IDA]
- intracellular protein transmembrane import [IMP]
- misfolded protein transport [IMP]
- posttranslational protein targeting to membrane, translocation [IDA, IMP]
- retrograde protein transport, ER to cytosol [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
VHS2
YIL135C
Regulator of septin dynamics; involved in the regulation of septin dynamics at bud neck after mitotic entry, likely by stabilizing septin structure; regulated at post-translational level by cell cycle dependent phosphorylation; likely phosphorylated by Cdc28p and dephosphorylated by Cdc14p before cytokinesis; high-copy suppressor of synthetic lethality of sis2 sit4 double mutant; VHS2 has a paralog, MLF3, that arose from the whole genome duplication
GO Process (1)
GO Function (0)
GO Component (1)
Gene Ontology Biological Process
Saccharomyces cerevisiae (S288c)
Co-localization
Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.
Publication
A systematic proximity ligation approach to studying protein-substrate specificity identifies the substrate spectrum of the Ssh1 translocon.
Many cellular functions are carried out by protein pairs or families, providing robustness alongside functional diversity. For such processes, it remains a challenge to map the degree of specificity versus promiscuity. Protein-protein interactions (PPIs) can be used to inform on these matters as they highlight cellular locals, regulation and, in cases where proteins affect other proteins - substrate range. However, ... [more]
EMBO J Jun. 01, 2023; 42(11);e113385 [Pubmed: 37073826]
Throughput
- High Throughput
Additional Notes
- Cel-lctiv (CELlular biotin-Ligation for Capturing Transient Interactions in vivo)
- Protein that bound both Sec61p and Ssh1p with no preference for either protein.
- Proximity ligation assay
Curated By
- BioGRID