BAIT

RAB4A

HRES-1, HRES-1/RAB4, HRES1, RAB4
RAB4A, member RAS oncogene family
Homo sapiens
PREY

LARS

HSPC192, ILFS1, LARS1, LEURS, LEUS, LFIS, LRS, PIG44, RNTLS, hr025Cl
leucyl-tRNA synthetase
GO Process (4)
GO Function (3)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Proximity labelling identifies pro-migratory endocytic recycling cargo and machinery of the Rab4 and Rab11 families.

Wilson B, Flett C, Gemperle J, Lawless C, Hartshorn M, Hinde E, Harrison T, Chastney M, Taylor S, Allen J, Norman JC, Zacharchenko T, Caswell PT

Endocytic recycling controls the return of internalised cargoes to the plasma membrane to coordinate their positioning, availability and downstream signalling. The Rab4 and Rab11 small GTPase families regulate distinct recycling routes, broadly classified as fast recycling from early endosomes (Rab4) and slow recycling from perinuclear recycling endosomes (Rab11), and both routes handle a broad range of overlapping cargoes to regulate ... [more]

J Cell Sci Jun. 15, 2023; 136(12); [Pubmed: 37232246]

Throughput

  • High Throughput

Additional Notes

  • BioID
  • Greater than 2-fold enrichment, at least two unique peptides, in at least three of four repeats
  • Prey protein is part of longlist

Curated By

  • BioGRID