An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Defining distinct RNA-protein interactomes of SARS-CoV-2 genomic and subgenomic RNAs

Whitworth IT, Knoener R, Puray Chavez M, Halfmann P, Romero S, Baddouh M, Scalf M, Kawaoka Y, Kutluay SB, Smith LM, Sherer NM

Host RNA binding proteins recognize viral RNA and play key roles in virus replication and antiviral defense mechanisms. SARS-CoV-2 generates a series of tiered subgenomic RNAs (sgRNAs), each encoding distinct viral protein(s) that regulate different aspects of viral replication. Here, for the first time, we demonstrate the successful isolation of SARS-CoV-2 genomic RNA and three distinct sgRNAs (N, S, and ... [more]

Status: Preliminary Report

Throughput

High Throughput

Additional Notes

24 hours post infection (24-hpi) with SARS-CoV-2
SARS-CoV-2 RNA and human protein interaction in Huh7+AT cells modified to express the SARS-CoV-2 receptor ACE2 and entry co-factor TMPRSS2.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
N MTHFD1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Nabeel-Shah S (2022)	High	-	BioGRID	3660509
N MTHFD1	Affinity Capture-RNA Affinity Capture-RNA An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.	Whitworth IT (2024)	High	-	BioGRID	3777927
N MTHFD1	Protein-RNA Protein-RNA An interaction is detected between and protein and an RNA in vitro.	Jiang L (2023)	High	-	BioGRID	3749082

Curated By

BioGRID

Interaction Summary

N

MTHFD1

Gene Ontology Biological Process

Gene Ontology Molecular Function

formate-tetrahydrofolate ligase activity [IBA, TAS]methenyltetrahydrofolate cyclohydrolase activity [IBA, TAS]methylenetetrahydrofolate dehydrogenase (NADP+) activity [IBA]methylenetetrahydrofolate dehydrogenase [NAD(P)+] activity [TAS]

Gene Ontology Cellular Component

Affinity Capture-RNA