BAIT

N

nuc, nucleocapsid, SARS-CoV2 N, N protein, ncap, SARS-CoV-2 N, Nucleoprotein, NCAP_SARS2, PRO_0000449656, GU280_gp10
Nucleocapsid phosphoprotein; Nucleoprotein
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

GPR112

PGR17, RP1-299I16, RP11-51D15.1
G protein-coupled receptor 112
GO Process (1)
GO Function (0)
GO Component (0)

Gene Ontology Biological Process

Homo sapiens

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Defining distinct RNA-protein interactomes of SARS-CoV-2 genomic and subgenomic RNAs

Whitworth IT, Knoener R, Puray Chavez M, Halfmann P, Romero S, Baddouh M, Scalf M, Kawaoka Y, Kutluay SB, Smith LM, Sherer NM

Host RNA binding proteins recognize viral RNA and play key roles in virus replication and antiviral defense mechanisms. SARS-CoV-2 generates a series of tiered subgenomic RNAs (sgRNAs), each encoding distinct viral protein(s) that regulate different aspects of viral replication. Here, for the first time, we demonstrate the successful isolation of SARS-CoV-2 genomic RNA and three distinct sgRNAs (N, S, and ... [more]

Status: Preliminary Report

Throughput

  • High Throughput

Additional Notes

  • 24 hours post infection (24-hpi) with SARS-CoV-2
  • SARS-CoV-2 RNA and human protein interaction in Huh7+AT cells modified to express the SARS-CoV-2 receptor ACE2 and entry co-factor TMPRSS2.

Curated By

  • BioGRID