Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

SUMOylation of ERp44 enhances Ero1? ER retention contributing to the pathogenesis of obesity and insulin resistance.

Xie H, Wang YH, Liu X, Gao J, Yang C, Huang T, Zhang L, Luo X, Gao Z, Wang T, Yan T, Liu Y, Yang P, Yu Q, Liu S, Wang Y, Xiong F, Zhang S, Zhou Q, Wang CY

As the only E2 conjugating enzyme for the SUMO system, Ubc9-mediated SUMOylation has been recognized to regulate diverse biological processes, but its impact on adipocytes relevant to obesity and insulin resistance is yet to be elucidated.We established adipocyte-specific Ubc9 deficient mice to explore the effects of Ubc9 on obesity and metabolic disorders induced by high-fat diet (HFD) in adult mice. ... [more]

Metabolism Feb. 01, 2023; 139();155351 [Pubmed: 36427672]

Throughput

  • Low Throughput

Curated By

  • BioGRID