RPT6
Gene Ontology Biological Process
- chromatin remodeling [IMP]
- negative regulation of sequence-specific DNA binding transcription factor activity [IMP]
- nonfunctional rRNA decay [IMP]
- nucleotide-excision repair [IGI]
- positive regulation of RNA polymerase II transcriptional preinitiation complex assembly [IGI, IMP]
- positive regulation of sequence-specific DNA binding transcription factor activity [IMP]
- positive regulation of transcription elongation from RNA polymerase II promoter [IMP]
- proteasome regulatory particle assembly [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IPI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PUP1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Involvement of Bag6 and the TRC pathway in proteasome assembly.
The 26S proteasome has an elaborate structure, consisting of 33 different subunits that form the 20S core particle capped by the 19S regulatory particle on either end. Several chaperones that are dedicated to the accurate assembly of this protease complex have been identified, but the mechanisms underlying proteasome biogenesis remain unexplored so far. Here we report that core particle assembly ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RPT6 PUP1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 7 | BioGRID | 3614630 | |
| PUP1 RPT6 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.1287 | BioGRID | 1952516 | |
| PUP1 RPT6 | Positive Genetic Positive Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores. | High | 0.043 | BioGRID | 443103 | |
| RPT6 PUP1 | Positive Genetic Positive Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores. | High | 0.043 | BioGRID | 443104 | |
| RPT6 PUP1 | Positive Genetic Positive Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores. | High | 2.9647 | BioGRID | 901705 |
Curated By
- BioGRID