BAIT

MUM2

SPOT8, L000003595, L000002013, YBR057C
Protein essential for meiotic DNA replication and sporulation; cytoplasmic protein; subunit of the MIS complex which controls mRNA methylation during during the induction of sporulation; also interacts with Orc2p, which is a component of the origin recognition complex
GO Process (4)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

SLZ1

L000004192, YNL196C
Sporulation-specific protein with a leucine zipper motif; subunit of the MIS complex which controls mRNA methylation during during the induction of sporulation
GO Process (3)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The yeast RNA methylation complex consists of conserved yet reconfigured components with m6A-dependent and independent roles.

Ensinck I, Maman A, Albihlal WS, Lassandro M, Salzano G, Sideri T, Howell SA, Calvani E, Patel H, Bushkin G, Ralser M, Snijders AP, Skehel M, Casanal A, Schwartz S, van Werven FJ

N6-methyladenosine (m6A), the most abundant mRNA modification, is deposited in mammals/insects/plants by m6A methyltransferase complexes (MTC) comprising a catalytic subunit and at least five additional proteins. The yeast MTC is critical for meiosis and was known to comprise three proteins, of which two were conserved. We uncover three novel MTC components (Kar4/Ygl036w-Vir1/Dyn2). All MTC subunits, except for Dyn2, are essential ... [more]

Elife Jul. 25, 2023; 12(); [Pubmed: 37490041]

Throughput

  • Low Throughput

Curated By

  • BioGRID