Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

The E3 ligase subunit FBXO45 binds the interferon-? receptor and promotes its degradation during influenza virus infection.

Tsai M, Osman W, Adair J, ElMergawy R, Chafin L, Johns F, Farkas D, Elhance A, Londino J, Mallampalli RK

Influenza remains a major public health challenge, as the viral infection activates multiple biological networks linked to altered host innate immunity. Following infection, IFN-?, a ligand crucial for the resolution of viral infections, is known to bind to its cognate receptor, IFNLR1, in lung epithelia. However, little is known regarding the molecular expression and regulation of IFNLR1. Here, we show ... [more]

J Biol Chem Dec. 01, 2022; 298(12);102698 [Pubmed: 36379255]

Throughput

  • High Throughput

Curated By

  • BioGRID