NEDD4
Gene Ontology Biological Process
- cellular response to UV [IMP]
- cytokine-mediated signaling pathway [TAS]
- development involved in symbiotic interaction [IMP]
- glucocorticoid receptor signaling pathway [IDA]
- lysosomal transport [IDA]
- negative regulation of sodium ion transport [IDA]
- negative regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage [IMP]
- negative regulation of vascular endothelial growth factor receptor signaling pathway [ISS]
- neuron projection development [IEP]
- positive regulation of nucleocytoplasmic transport [IDA]
- positive regulation of phosphatidylinositol 3-kinase signaling [IMP]
- positive regulation of protein catabolic process [IDA]
- progesterone receptor signaling pathway [IDA]
- protein K63-linked ubiquitination [ISS]
- protein targeting to lysosome [IDA]
- protein ubiquitination [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IDA, IMP]
- receptor catabolic process [IDA]
- receptor internalization [IDA]
- regulation of dendrite morphogenesis [ISS]
- regulation of ion transmembrane transport [IDA]
- regulation of membrane potential [IDA]
- regulation of potassium ion transmembrane transporter activity [IDA]
- response to calcium ion [TAS]
- transmission of virus [IMP]
- ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [IMP]
Gene Ontology Molecular Function- RNA polymerase binding [IPI]
- beta-2 adrenergic receptor binding [IDA]
- phosphoserine binding [ISS]
- phosphothreonine binding [ISS]
- proline-rich region binding [IMP, IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- sodium channel inhibitor activity [IDA]
- ubiquitin binding [IDA]
- ubiquitin-protein transferase activity [IDA]
- RNA polymerase binding [IPI]
- beta-2 adrenergic receptor binding [IDA]
- phosphoserine binding [ISS]
- phosphothreonine binding [ISS]
- proline-rich region binding [IMP, IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- sodium channel inhibitor activity [IDA]
- ubiquitin binding [IDA]
- ubiquitin-protein transferase activity [IDA]
Gene Ontology Cellular Component
UBE2D3
Gene Ontology Biological Process
- BMP signaling pathway [TAS]
- MyD88-independent toll-like receptor signaling pathway [TAS]
- TRIF-dependent toll-like receptor signaling pathway [TAS]
- cellular protein modification process [TAS]
- cellular response to hypoxia [TAS]
- gene expression [TAS]
- innate immune response [TAS]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- negative regulation of type I interferon production [TAS]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IDA]
- protein K11-linked ubiquitination [IDA]
- protein K48-linked ubiquitination [IDA]
- protein monoubiquitination [IDA]
- protein polyubiquitination [IDA]
- protein ubiquitination [IDA]
- regulation of transcription from RNA polymerase II promoter in response to hypoxia [TAS]
- toll-like receptor 3 signaling pathway [TAS]
- toll-like receptor 4 signaling pathway [TAS]
- toll-like receptor signaling pathway [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [TAS]
- ubiquitin-dependent protein catabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
Monitoring HECT Ubiquitination Activity In Vitro.
In vitro ubiquitination tools have been employed to mechanistically study the ubiquitin enzymatic cascade. Here, we describe an assay capable to monitor ubiquitin conjugation in real time using the Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) system. The assay requires purified E1 and E2 enzymes, the HECT E3 ligase of choice and two fluorophore-labeled ubiquitins. This single step technique represents an ... [more]
Throughput
- Low Throughput
Additional Notes
- The reaction contained UBA1 [E1], UBE2D3 [E2], and NEDD4 [E3]
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| UBE2D3 NEDD4 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 263697 | |
| UBE2D3 NEDD4 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 1067163 | |
| NEDD4 UBE2D3 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 2971708 | |
| NEDD4 UBE2D3 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 1526331 | |
| NEDD4 UBE2D3 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 937881 | |
| NEDD4 UBE2D3 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| NEDD4 UBE2D3 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 2491288 | |
| NEDD4 UBE2D3 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 1513480 | |
| NEDD4 UBE2D3 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| UBE2D3 NEDD4 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 872385 | |
| NEDD4 UBE2D3 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 1067293 | |
| NEDD4 UBE2D3 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 1523835 | |
| NEDD4 UBE2D3 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 1065887 | |
| NEDD4 UBE2D3 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| NEDD4 UBE2D3 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 330008 | |
| NEDD4 UBE2D3 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| NEDD4 UBE2D3 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 1029241 |
Curated By
- BioGRID