An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Genome-wide CRISPR-cas9 knockout screening identifies GRB7 as a driver for MEK inhibitor resistance in KRAS mutant colon cancer.

Yu C, Luo D, Yu J, Zhang M, Zheng X, Xu G, Wang J, Wang H, Xu Y, Jiang K, Xu J, Ma X, Jing J, Shi H

Targeting the KRAS pathway is a promising but challenging approach for colorectal cancer therapy. Despite showing potent efficacy in BRAF-mutated melanoma, MEK inhibitors appeared to be tolerated by colorectal cancer cells due to their intrinsic compensatory signaling. Here, we performed genome-wide CRISPR/Cas9 screening in the presence of MEK inhibitor to identify genes that are synthetically lethal with MEK inhibition in ... [more]

Oncogene Jan. 01, 2022; 41(2);191-203 [Pubmed: 34718347]

Throughput

Low Throughput

Additional Notes

hit genes enriched at least 3-fold over n HCT116 cells expressing FLAG-GRB7 compared to that in control cells

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
GRB7 RPL31	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Kennedy SA (2020)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

GRB7

Gene Ontology Biological Process

Gene Ontology Molecular Function

SH3/SH2 adaptor activity [TAS]identical protein binding [IPI]phosphatidylinositol binding [IDA]protein binding [IPI]protein kinase binding [IDA]

Gene Ontology Cellular Component

RPL31

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA binding [TAS]poly(A) RNA binding [IDA]structural constituent of ribosome [NAS]