An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Mapping the MOB proteins' proximity network reveals a unique interaction between human MOB3C and the RNase P complex.

Elkholi IE, Boulais J, Thibault MP, Phan HD, Robert A, Lai LB, Faubert D, Smith MJ, Gopalan V, Cote JF

Distinct functions mediated by members of the monopolar spindle-one-binder (MOB) family of proteins remain elusive beyond the evolutionarily conserved and well-established roles of MOB1 (MOB1A/B) in regulating tissue homeostasis within the Hippo pathway. Since MOB proteins are adaptors, understanding how they engage in protein-protein interactions, and help assemble complexes is essential to define the full scope of their biological functions. ... [more]

J Biol Chem Aug. 01, 2023; ();105123 [Pubmed: 37536630]

Throughput

High Throughput

Additional Notes

BFDR less than or equal to 0.01
BioID
Experiment performed in HEK293 cells

Curated By

BioGRID

Interaction Summary

MOB3A

Gene Ontology Cellular Component

AP3M1

Gene Ontology Biological Process

Gene Ontology Molecular Function

Rab GTPase binding [IPI]protein binding [IPI]