Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

An interaction between OTULIN and SCRIB uncovers roles for linear ubiquitination in planar cell polarity.

Almeida SM, Ivantsiv S, Niibori R, Dunham WH, Green BA, Zhao L, Gingras AC, Cordes SP

Planar cell polarity (PCP) plays critical roles in developmental and homeostatic processes. Membrane presentation of PCP complexes containing Van Gogh-like (VANGL) transmembrane proteins is central to PCP and can be directed by the scaffold protein scribble (SCRIB). The role atypical linear ubiquitin (Met1-Ub) chains might play in PCP is unknown. Here, HEK293 cell-based interactomic analyses of the Met1-Ub deubiquitinase OTULIN ... [more]

Dis Model Mech Aug. 01, 2023; 16(8); [Pubmed: 37589075]

Throughput

  • High Throughput

Additional Notes

  • High confidence interactors met a SAINT threshold > 0.8 in the AP-MS analysis of at least one FLAG-tagged OTULIN cell line.
  • OTULIN delta-PBM construct

Curated By

  • BioGRID