An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Self-association of Puralpha is mediated by RNA.

Gallia GL, Darbinian N, Johnson EM, Khalili K

The 322 amino acid cellular protein, Puralpha, is a sequence-specific single-stranded DNA-binding protein implicated in control of transcription and replication. Previous studies have demonstrated that the interaction between Puralpha and its target DNA sequence results in the formation of multimeric complexes. In this study, we demonstrate that Puralpha can self-associate in the absence of DNA. This self-association, while independent of ... [more]

J Cell Biochem Sep. 01, 1999; 74(3);334-48 [Pubmed: 10412036]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

PURA

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [TAS]double-stranded telomeric DNA binding [IDA]poly(A) RNA binding [IDA]protein binding [IPI]single-stranded DNA binding [ISS]transcription factor binding [ISS]

Gene Ontology Cellular Component