BAIT

SGO1

YOR29-24, YOR073W
Component of the spindle checkpoint; involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability; recruits condensin to the pericentric region of chromosomes during meiosis; dissociates from pericentromeres when sister kinetochores are under tension
Saccharomyces cerevisiae (S288c)
PREY

MAD2

spindle checkpoint protein MAD2, L000000975, YJL030W
Component of the spindle-assembly checkpoint complex; delays onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability
GO Process (3)
GO Function (0)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

SUMOylation stabilizes sister kinetochore biorientation to allow timely anaphase.

Su XB, Wang M, Schaffner C, Nerusheva OO, Clift D, Spanos C, Kelly DA, Tatham M, Wallek A, Wu Y, Rappsilber J, Jeyaprakash AA, Storchova Z, Hay RT, Marston AL

During mitosis, sister chromatids attach to microtubules from opposite poles, called biorientation. Sister chromatid cohesion resists microtubule forces, generating tension, which provides the signal that biorientation has occurred. How tension silences the surveillance pathways that prevent cell cycle progression and correct erroneous kinetochore-microtubule attachments remains unclear. Here we show that SUMOylation dampens error correction to allow stable sister kinetochore biorientation ... [more]

J Cell Biol Jul. 05, 2021; 220(7); [Pubmed: 33929514]

Throughput

  • Low Throughput

Ontology Terms

  • cell cycle progression (APO:0000253)

Additional Notes

  • deletion of the SAC component, MAD2, rescued the metaphase delay of siz1/siz2 and sgo1-4R cells

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SGO1 MAD2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-2.9218BioGRID
222875
SGO1 MAD2
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Low-BioGRID
162506

Curated By

  • BioGRID