BAIT

SGO1

YOR29-24, YOR073W
Component of the spindle checkpoint; involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability; recruits condensin to the pericentric region of chromosomes during meiosis; dissociates from pericentromeres when sister kinetochores are under tension
Saccharomyces cerevisiae (S288c)
PREY

TUB3

alpha-tubulin TUB3, L000002389, YML124C
Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

SUMOylation stabilizes sister kinetochore biorientation to allow timely anaphase.

Su XB, Wang M, Schaffner C, Nerusheva OO, Clift D, Spanos C, Kelly DA, Tatham M, Wallek A, Wu Y, Rappsilber J, Jeyaprakash AA, Storchova Z, Hay RT, Marston AL

During mitosis, sister chromatids attach to microtubules from opposite poles, called biorientation. Sister chromatid cohesion resists microtubule forces, generating tension, which provides the signal that biorientation has occurred. How tension silences the surveillance pathways that prevent cell cycle progression and correct erroneous kinetochore-microtubule attachments remains unclear. Here we show that SUMOylation dampens error correction to allow stable sister kinetochore biorientation ... [more]

J Cell Biol Jul. 05, 2021; 220(7); [Pubmed: 33929514]

Throughput

  • High Throughput

Additional Notes

  • Co-IP with Sgo1-4R or Sgo1

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SGO1 TUB3
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-3.3092BioGRID
221428

Curated By

  • BioGRID