BAIT

KCNH1

potassium voltage-gated channel, subfamily H (eag-related), member 1
GO Process (2)
GO Function (2)
GO Component (4)
Rattus norvegicus

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

14-3-3 proteins regulate cullin 7-mediated Eag1 degradation.

Hsieh CH, Chou CC, Fang YC, Hsu PH, Chiu YH, Yang CS, Jow GM, Tang CY, Jeng CJ

Mutations in the human gene encoding the neuron-specific Eag1 (KV10.1; KCNH1) potassium channel are linked to congenital neurodevelopmental diseases. Disease-causing mutant Eag1 channels manifest aberrant gating function and defective protein homeostasis. Both the E3 ubiquitin ligase cullin 7 (Cul7) and the small acid protein 14-3-3 serve as binding partners of Eag1. Cul7 mediates proteasomal and lysosomal degradation of Eag1 protein, ... [more]

Cell Biosci Jan. 30, 2023; 13(1);18 [Pubmed: 36717938]

Throughput

  • Low Throughput

Curated By

  • BioGRID