PREY

KCNH1

EAG, EAG1, Kv10.1, h-eag
potassium voltage-gated channel, subfamily H (eag-related), member 1
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

14-3-3 proteins regulate cullin 7-mediated Eag1 degradation.

Hsieh CH, Chou CC, Fang YC, Hsu PH, Chiu YH, Yang CS, Jow GM, Tang CY, Jeng CJ

Mutations in the human gene encoding the neuron-specific Eag1 (KV10.1; KCNH1) potassium channel are linked to congenital neurodevelopmental diseases. Disease-causing mutant Eag1 channels manifest aberrant gating function and defective protein homeostasis. Both the E3 ubiquitin ligase cullin 7 (Cul7) and the small acid protein 14-3-3 serve as binding partners of Eag1. Cul7 mediates proteasomal and lysosomal degradation of Eag1 protein, ... [more]

Cell Biosci Jan. 30, 2023; 13(1);18 [Pubmed: 36717938]

Throughput

  • Low Throughput

Curated By

  • BioGRID