An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Flotillin-2 regulates epidermal growth factor receptor activation, degradation by Cbl-mediated ubiquitination, and cancer growth.

Wisniewski DJ, Liyasova MS, Korrapati S, Zhang X, Ratnayake S, Chen Q, Gilbert SF, Catalano A, Voeller D, Meerzaman D, Guha U, Porat-Shliom N, Annunziata CM, Lipkowitz S

Epidermal growth factor receptor (EGFR) signaling is frequently dysregulated in various cancers. The ubiquitin ligase Casitas B-lineage lymphoma proto-oncogene (Cbl) regulates degradation of activated EGFR through ubiquitination and acts as an adaptor to recruit proteins required for trafficking. Here, we used stable isotope labeling with amino acids in cell culture mass spectrometry to compare Cbl complexes with or without epidermal ... [more]

J Biol Chem Jan. 01, 2023; 299(1);102766 [Pubmed: 36470425]

Throughput

Low Throughput

Additional Notes

Proximity Ligation Assay

Curated By

BioGRID

Interaction Summary

CBL

Gene Ontology Biological Process

Gene Ontology Molecular Function

SH3 domain binding [IPI]ephrin receptor binding [IPI]protein binding [IPI]sequence-specific DNA binding transcription factor activity [TAS]ubiquitin-protein transferase activity [TAS]

Gene Ontology Cellular Component

TUBB2A