BAIT

TOM70

MAS70, MOM72, OMP1, protein channel TOM70, L000001030, L000003141, YNL121C
Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)
PREY

CRD1

CLS1, cardiolipin synthase, L000003358, YDL142C
Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function; required to maintain tubular mitochondrial morphology and functions in mitochondrial fusion; also required for normal vacuolar ion homeostasis
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

The chaperone-binding activity of the mitochondrial surface receptor Tom70 protects the cytosol against mitoprotein-induced stress.

Backes S, Bykov YS, Flohr T, Raeschle M, Zhou J, Lenhard S, Kraemer L, Muehlhaus T, Bibi C, Jann C, Smith JD, Steinmetz LM, Rapaport D, Storchova Z, Schuldiner M, Boos F, Herrmann JM

Most mitochondrial proteins are synthesized as precursors in the cytosol and post-translationally transported into mitochondria. The mitochondrial surface protein Tom70 acts at the interface of the cytosol and mitochondria. In vitro import experiments identified Tom70 as targeting receptor, particularly for hydrophobic carriers. Using in vivo methods and high-content screens, we revisit the question of Tom70 function and considerably expand the set of ... [more]

Cell Rep Apr. 06, 2021; 35(1);108936 [Pubmed: 33826901]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: colony size (APO:0000063)

Additional Notes

  • SGA, threshold 50% reduction in growth in tom70 deletion strain but not TOM70wt

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TOM70 CRD1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1711BioGRID
2169726
TOM70 CRD1
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Low-BioGRID
353772

Curated By

  • BioGRID