Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Regulation of ATR activity via the RNA polymerase II associated factors CDC73 and PNUTS-PP1.

Landsverk HB, Sandquist LE, Sridhara SC, Rodland GE, Sabino JC, de Almeida SF, Grallert B, Trinkle-Mulcahy L, Syljuasen RG

Ataxia telangiectasia mutated and Rad3-related (ATR) kinase is a key factor activated by DNA damage and replication stress. An alternative pathway for ATR activation has been proposed to occur via stalled RNA polymerase II (RNAPII). However, how RNAPII might signal to activate ATR remains unknown. Here, we show that ATR signaling is increased after depletion of the RNAPII phosphatase PNUTS-PP1, ... [more]

Nucleic Acids Res Feb. 28, 2019; 47(4);1797-1813 [Pubmed: 30541148]

Throughput

  • Low Throughput

Curated By

  • BioGRID