A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

LRP8-mediated selenocysteine uptake is a targetable vulnerability in MYCN-amplified neuroblastoma.

Alborzinia H, Chen Z, Yildiz U, Freitas FP, Vogel FCE, Varga JP, Batani J, Bartenhagen C, Schmitz W, Buechel G, Michalke B, Zheng J, Meierjohann S, Girardi E, Espinet E, Florez AF, Dos Santos AF, Aroua N, Cheytan T, Haenlin J, Schlicker L, Xavier da Silva TN, Przybylla A, Zeisberger P, Superti-Furga G, Eilers M, Conrad M, Fabiano M, Schweizer U, Fischer M, Schulze A, Trumpp A, Friedmann Angeli JP

Ferroptosis has emerged as an attractive strategy in cancer therapy. Understanding the operational networks regulating ferroptosis may unravel vulnerabilities that could be harnessed for therapeutic benefit. Using CRISPR-activation screens in ferroptosis hypersensitive cells, we identify the selenoprotein P (SELENOP) receptor, LRP8, as a key determinant protecting MYCN-amplified neuroblastoma cells from ferroptosis. Genetic deletion of LRP8 leads to ferroptosis as a ... [more]

EMBO Mol Med Jul. 12, 2023; ();e18014 [Pubmed: 37435859]

Throughput

Low Throughput

Additional Notes

CRISPR-based genetic interaction

Curated By

BioGRID

Interaction Summary

LRP8

Gene Ontology Biological Process

Gene Ontology Molecular Function

apolipoprotein binding [IC]protein binding [IPI]reelin receptor activity [ISS]transmembrane signaling receptor activity [TAS]very-low-density lipoprotein particle receptor activity [IDA]

Gene Ontology Cellular Component

SLC31A1