An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

METTL3 protects METTL14 from STUB1-mediated degradation to maintain m6 A homeostasis.

Zeng ZC, Pan Q, Sun YM, Huang HJ, Chen XT, Chen TQ, He B, Ye H, Zhu SX, Pu KJ, Fang K, Huang W, Chen YQ, Wang WT

N6 -Methyladenosine (m6 A) is an important RNA modification catalyzed by methyltransferase-like 3 (METTL3) and METTL14. m6 A homeostasis mediated by the methyltransferase (MTase) complex plays key roles in various biological processes. However, the mechanism underlying METTL14 protein stability and its role in m6 A homeostasis remain elusive. Here, we show that METTL14 stability is regulated by the competitive interaction ... [more]

EMBO Rep Mar. 06, 2023; 24(3);e55762 [Pubmed: 36597993]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
METTL14 PRKDC	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Yue Y (2018)	High	-	BioGRID	2566239

Curated By

BioGRID

Interaction Summary

METTL14

Gene Ontology Biological Process

Gene Ontology Molecular Function

mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity [IDA]

Gene Ontology Cellular Component

PRKDC