BAIT
PARK2
Park, Prkn
parkin RBR E3 ubiquitin protein ligase
GO Process (63)
GO Function (22)
GO Component (22)
Gene Ontology Biological Process
- adult locomotory behavior [ISO, ISS]
- aggresome assembly [ISO, ISS]
- cellular protein catabolic process [ISO]
- cellular protein modification process [TAS]
- cellular response to toxic substance [ISO, ISS]
- dopamine metabolic process [ISO]
- dopamine uptake involved in synaptic transmission [ISO]
- learning [ISO]
- locomotory behavior [ISO]
- mitochondrial fission [ISS]
- mitochondrion degradation [ISO, ISS]
- mitochondrion organization [ISS]
- negative regulation of JNK cascade [ISS]
- negative regulation of actin filament bundle assembly [ISO]
- negative regulation of cell death [ISO]
- negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway [ISO, ISS]
- negative regulation of glucokinase activity [ISO, ISS]
- negative regulation of insulin secretion [ISO, ISS]
- negative regulation of mitochondrial fusion [ISS]
- negative regulation of neuron apoptotic process [ISO, ISS]
- negative regulation of neuron death [IGI, ISO]
- negative regulation of oxidative stress-induced neuron death [IGI]
- negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway [ISO, ISS]
- negative regulation of protein phosphorylation [ISO, ISS]
- negative regulation of reactive oxygen species metabolic process [ISO, ISS]
- negative regulation of release of cytochrome c from mitochondria [ISO, ISS]
- neuron cellular homeostasis [ISS]
- neuron maturation [IEP]
- norepinephrine metabolic process [ISO]
- positive regulation of DNA binding [ISO, ISS]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [ISO, ISS]
- positive regulation of mitochondrial fission [ISS]
- positive regulation of mitochondrial fusion [ISO, ISS]
- positive regulation of proteasomal protein catabolic process [ISO]
- positive regulation of protein linear polyubiquitination [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO, ISS]
- positive regulation of tumor necrosis factor-mediated signaling pathway [ISO, ISS]
- proteasome-mediated ubiquitin-dependent protein catabolic process [ISO, ISS]
- protein K48-linked ubiquitination [ISO, ISS]
- protein K63-linked ubiquitination [ISO, ISS]
- protein autoubiquitination [ISO, ISS]
- protein localization to mitochondrion [ISS]
- protein metabolic process [ISO]
- protein monoubiquitination [ISO, ISS]
- protein polyubiquitination [ISO, ISS]
- protein ubiquitination [ISO, ISS]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [ISO, ISS]
- regulation of autophagy [ISO, ISS]
- regulation of cellular response to oxidative stress [ISS]
- regulation of mitochondrion organization [ISO, ISS]
- regulation of neurotransmitter secretion [ISO]
- regulation of reactive oxygen species metabolic process [ISO]
- regulation of synaptic transmission [ISO]
- response to corticosterone [IEP]
- response to drug [IEP]
- response to endoplasmic reticulum stress [ISO]
- response to oxidative stress [ISS]
- response to unfolded protein [IDA]
- startle response [ISO]
- synaptic transmission, dopaminergic [ISO]
- synaptic transmission, glutamatergic [ISO]
- ubiquitin-dependent protein catabolic process [TAS]
- zinc ion homeostasis [ISS]
Gene Ontology Molecular Function- F-box domain binding [ISO, ISS]
- G-protein coupled receptor binding [ISO, ISS]
- Hsp70 protein binding [ISO, ISS]
- PDZ domain binding [ISO, ISS]
- actin binding [ISO]
- chaperone binding [ISO]
- cullin family protein binding [ISO, ISS]
- heat shock protein binding [ISO]
- histone deacetylase binding [ISO]
- identical protein binding [ISO, ISS]
- kinase binding [ISO]
- protein binding [IPI]
- protein complex binding [IDA]
- protein kinase binding [ISO, ISS]
- transcription regulatory region sequence-specific DNA binding [ISS]
- tubulin binding [ISO]
- ubiquitin binding [ISO, ISS]
- ubiquitin conjugating enzyme binding [ISO, ISS]
- ubiquitin protein ligase activity [ISO]
- ubiquitin protein ligase binding [ISO]
- ubiquitin-protein transferase activity [ISO, ISS, TAS]
- ubiquitin-specific protease binding [ISO]
- F-box domain binding [ISO, ISS]
- G-protein coupled receptor binding [ISO, ISS]
- Hsp70 protein binding [ISO, ISS]
- PDZ domain binding [ISO, ISS]
- actin binding [ISO]
- chaperone binding [ISO]
- cullin family protein binding [ISO, ISS]
- heat shock protein binding [ISO]
- histone deacetylase binding [ISO]
- identical protein binding [ISO, ISS]
- kinase binding [ISO]
- protein binding [IPI]
- protein complex binding [IDA]
- protein kinase binding [ISO, ISS]
- transcription regulatory region sequence-specific DNA binding [ISS]
- tubulin binding [ISO]
- ubiquitin binding [ISO, ISS]
- ubiquitin conjugating enzyme binding [ISO, ISS]
- ubiquitin protein ligase activity [ISO]
- ubiquitin protein ligase binding [ISO]
- ubiquitin-protein transferase activity [ISO, ISS, TAS]
- ubiquitin-specific protease binding [ISO]
Gene Ontology Cellular Component
- Golgi apparatus [ISO]
- Golgi membrane [IDA]
- LUBAC complex [ISO, ISS]
- Parkin-FBXW7-Cul1 ubiquitin ligase complex [ISO, ISS]
- SCF ubiquitin ligase complex [ISO]
- aggresome [ISO, ISS]
- cytoplasm [IDA, ISO, ISS]
- cytoplasmic vesicle [IDA]
- cytosol [ISO, ISS]
- endoplasmic reticulum [IDA, ISO]
- endoplasmic reticulum membrane [IDA]
- intracellular [IDA]
- membrane raft [IDA]
- mitochondrion [ISO, ISS]
- neuron projection [ISO]
- nucleus [ISO]
- perinuclear region of cytoplasm [ISO]
- postsynaptic density [IDA]
- protein complex [ISO]
- synapse [IDA]
- synaptic vesicle [IDA]
- ubiquitin ligase complex [ISO, ISS]
Rattus norvegicus
PREY
CISD1
C10orf70, ZCD1, mitoNEET, MDS029
CDGSH iron sulfur domain 1
GO Process (0)
GO Function (0)
GO Component (2)
Gene Ontology Cellular Component
Homo sapiens
Biochemical Activity (Ubiquitination)
An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.
Publication
Elucidation of ubiquitin-conjugating enzymes that interact with RBR-type ubiquitin ligases using a liquid-liquid phase separation-based method.
RING-between RING (RBR)-type ubiquitin (Ub) ligases (E3s) such as Parkin receive Ub from Ub-conjugating enzymes (E2s) in response to ligase activation. However, the specific E2s that transfer Ub to each RBR-type ligase are largely unknown because of insufficient methods for monitoring their interaction. To address this problem, we have developed a method that detects intracellular interactions between E2s and activated ... [more]
J Biol Chem Feb. 01, 2023; 299(2);102822 [Pubmed: 36563856]
Throughput
- Low Throughput
Additional Notes
- E2: UBE2L3
Curated By
- BioGRID