BAIT

S

spike, SARS-CoV2 S, SARS-CoV2 spike, S protein, surface, SARS-CoV-2 spike, SARS-CoV-2 S, SPIKE_SARS2, PRO_0000449646, GU280_gp02
Spike glycoprotein; Surface glycoprotein
COVID-19 Coronavirus Project
Synthetic Protein Interaction Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

EXOSC7

EAP1, RRP42, Rrp42p, hRrp42p, p8
exosome component 7
Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

High-resolution photocatalytic mapping of SARS-CoV-2 spike interactions on the cell surface.

Datta S, Chen DY, Tavares AH, Reyes-Robles T, Ryu KA, Khan N, Bechtel TJ, Bertoch JM, White CH, Hazuda DJ, Vora KA, Hett EC, Fadeyi OO, Oslund RC, Emili A, Saeed M

Identifying virus-host interactions on the cell surface can improve our understanding of viral entry and pathogenesis. SARS-CoV-2, the causative agent of the COVID-19 disease, uses ACE2 as a receptor to enter cells. Yet the full repertoire of cell surface proteins that contribute to viral entry is unknown. We developed a photocatalyst-based viral-host protein microenvironment mapping platform (ViraMap) to probe the ... [more]

Cell Chem Biol Jul. 17, 2023; (); [Pubmed: 37499664]

Throughput

  • High Throughput

Additional Notes

  • Bait: SARS-CoV-2 WT S protein
  • Enriched hits were identified with significant enrichment (log2FC greater than or equal to 1.5 and -log10p value of greater than or equal to 2 over control) and a SAINT BFDR of less than or equal to 0.01.
  • Photocatalyst-based viral-host protein microenvironment mapping platform (ViraMap).

Curated By

  • BioGRID