An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Pro-prion, as a membrane adaptor protein for E3 ligase c-Cbl, facilitates the ubiquitination of IGF-1R, promoting melanoma metastasis.

Li H, Zhang J, Ke JR, Yu Z, Shi R, Gao SS, Li JF, Gao ZX, Ke CS, Han HX, Xu J, Leng Q, Wu GR, Li Y, Tao L, Zhang X, Sy MS, Li C

Aberrant activation of receptor tyrosine kinase (RTK) is usually a result of mutation and plays important roles in tumorigenesis. How RTK without mutation affects tumorigenesis remains incompletely understood. Here we show that in human melanomas pro-prion (pro-PrP) is an adaptor protein for an E3 ligase c-Cbl, enabling it to polyubiquitinate activated insulin-like growth factor-1 receptor (IGF-1R), leading to enhanced melanoma ... [more]

Cell Rep Dec. 20, 2022; 41(12);111834 [Pubmed: 36543142]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

PRNP

Gene Ontology Biological Process

Gene Ontology Molecular Function

copper ion binding [IDA, TAS]identical protein binding [IPI]microtubule binding [IDA]protein binding [IPI]tubulin binding [IDA]

Gene Ontology Cellular Component

TMOD1