BAIT
RAB18
AA959686
RAB18, member RAS oncogene family
GO Process (5)
GO Function (3)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
RAB27A
GS2, HsT18676, RAB27, RAM
RAB27A, member RAS oncogene family
GO Process (21)
GO Function (4)
GO Component (8)
Gene Ontology Biological Process
- GTP catabolic process [IBA]
- Rab protein signal transduction [IBA]
- antigen processing and presentation [IMP]
- cellular protein metabolic process [TAS]
- complement-dependent cytotoxicity [IMP]
- exocytosis [IDA]
- exosomal secretion [IMP]
- intracellular protein transport [IBA]
- melanosome localization [IMP]
- melanosome transport [IBA]
- multivesicular body organization [IMP]
- multivesicular body sorting pathway [IMP]
- positive regulation of constitutive secretory pathway [IMP]
- positive regulation of exocytosis [IMP]
- positive regulation of gene expression [IMP]
- positive regulation of phagocytosis [IMP]
- positive regulation of reactive oxygen species biosynthetic process [IMP]
- positive regulation of regulated secretory pathway [IGI, IMP]
- protein secretion [IBA]
- synaptic vesicle transport [TAS]
- vesicle docking involved in exocytosis [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis.
Loss of functional RAB18 causes the autosomal recessive condition Warburg Micro syndrome. To better understand this disease, we used proximity biotinylation to generate an inventory of potential RAB18 effectors. A restricted set of 28 RAB18-interactions were dependent on the binary RAB3GAP1-RAB3GAP2 RAB18-guanine nucleotide exchange factor (GEF) complex. 12 of these 28 interactions are supported by prior reports and we have ... [more]
J Biol Chem Sep. 27, 2023; ();105295 [Pubmed: 37774976]
Throughput
- High Throughput
Additional Notes
- BioID
- HeLa cells
- Presence in 2 or more replicate experiments
Curated By
- BioGRID