BAIT

NSP14

ORF1ab, ORF1ab-nsp14, SARS-CoV2 nsp14, SARS-CoV-2 nsp14, Guanine-N7 methyltransferase, exonuclease, R1AB_SARS2, PRO_0000449631, GU280_gp01_nsp14
Guanine-N7 methyltransferase; Non-structural protein 14; 3-to-5 exonuclease
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

AHCY

SAHH, adoHcyase
adenosylhomocysteinase
Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

The proximal proteome of 17 SARS-CoV-2 proteins links to disrupted antiviral signaling and host translation.

Meyers JM, Ramanathan M, Shanderson RL, Beck A, Donohue L, Ferguson I, Guo MG, Rao DS, Miao W, Reynolds D, Yang X, Zhao Y, Yang YY, Blish C, Wang Y, Khavari PA

Viral proteins localize within subcellular compartments to subvert host machinery and promote pathogenesis. To study SARS-CoV-2 biology, we generated an atlas of 2422 human proteins vicinal to 17 SARS-CoV-2 viral proteins using proximity proteomics. This identified viral proteins at specific intracellular locations, such as association of accessary proteins with intracellular membranes, and projected SARS-CoV-2 impacts on innate immune signaling, ER-Golgi ... [more]

PLoS Pathog Oct. 01, 2021; 17(10);e1009412 [Pubmed: 34597346]

Throughput

  • High Throughput

Additional Notes

  • BioID
  • HEK293T cells
  • High confidence interactions had a SAINT score >= 0.9.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NSP14 AHCY
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High-BioGRID
3803672

Curated By

  • BioGRID