Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

INKILN is a Novel Long Noncoding RNA Promoting Vascular Smooth Muscle Inflammation via Scaffolding MKL1 and USP10.

Zhang W, Zhao J, Deng L, Ishimwe N, Pauli J, Wu W, Shan S, Kempf W, Ballantyne MD, Kim D, Lyu Q, Bennett M, Rodor J, Turner AW, Lu YW, Gao P, Choi M, Warthi G, Kim HW, Barroso MM, Bryant WB, Miller CL, Weintraub NL, Maegdefessel L, Miano JM, Baker AH, Long X

Activation of vascular smooth muscle cell (VSMC) inflammation is vital to initiate vascular disease. The role of human-specific long noncoding RNAs in VSMC inflammation is poorly understood.Bulk RNA sequencing in differentiated human VSMCs revealed a novel human-specific long noncoding RNA called inflammatory MKL1 (megakaryoblastic leukemia 1) interacting long noncoding RNA (INKILN). INKILN expression was assessed in multiple in vitro and ... [more]

Circulation Jul. 04, 2023; 148(1);47-67 [Pubmed: 37199168]

Throughput

Low Throughput

Additional Notes

Proximity Ligation Assay

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MKL1 USP10	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Zhang W (2023)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

MKL1

Gene Ontology Biological Process

Gene Ontology Molecular Function

actin binding [IDA]actin monomer binding [ISS]leucine zipper domain binding [IPI]protein binding [IPI]transcription coactivator activity [ISS]

Gene Ontology Cellular Component

USP10

Gene Ontology Biological Process

Gene Ontology Molecular Function

cysteine-type endopeptidase activity [IMP]ion channel binding [IDA]p53 binding [IPI]poly(A) RNA binding [IDA]protein binding [IPI]ubiquitin thiolesterase activity [IDA, IMP]ubiquitin-specific protease activity [IDA, IMP]