UBE2A
Gene Ontology Biological Process
- DNA repair [IGI]
- antigen processing and presentation of peptide antigen via MHC class I [TAS]
- histone H2A ubiquitination [IDA]
- positive regulation of cell proliferation [IDA]
- postreplication repair [NAS]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IBA]
- protein K11-linked ubiquitination [IDA]
- protein K48-linked ubiquitination [IDA]
- protein autoubiquitination [IDA]
- protein polyubiquitination [TAS]
- response to UV [IGI]
- ubiquitin-dependent protein catabolic process [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
UBE2B
Gene Ontology Biological Process
- DNA repair [IGI]
- canonical Wnt signaling pathway [ISS]
- cellular response to DNA damage stimulus [IDA]
- histone H2A ubiquitination [IMP]
- negative regulation of cAMP-mediated signaling [IDA]
- postreplication repair [IDA, NAS]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IDA]
- protein K11-linked ubiquitination [IDA]
- protein K48-linked ubiquitination [IDA]
- protein K63-linked ubiquitination [IDA]
- protein autoubiquitination [IDA]
- protein monoubiquitination [IMP]
- protein polyubiquitination [IMP]
- protein stabilization [IMP]
- protein ubiquitination [IDA]
- response to UV [IGI]
- response to drug [IDA]
- spermatogenesis [TAS]
- ubiquitin-dependent protein catabolic process [IDA, NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Paralog knockout profiling identifies DUSP4 and DUSP6 as a digenic dependence in MAPK pathway-driven cancers.
Although single-gene perturbation screens have revealed a number of new targets, vulnerabilities specific to frequently altered drivers have not been uncovered. An important question is whether the compensatory relationship between functionally redundant genes masks potential therapeutic targets in single-gene perturbation studies. To identify digenic dependencies, we developed a CRISPR paralog targeting library to investigate the viability effects of disrupting 3,284 ... [more]
Quantitative Score
- 5.48112e-05 [Confidence Score]
Throughput
- High Throughput
Additional Notes
- CRISPR GI screen
- Cell Line: MELJUSO_SKIN score (0.004309104593673)
- Cell Line: PATU8988S_PANCREAS score (4.16444587063914E-12)
- Cell Line: PK1_PANCREAS score (3.47680693002135E-26)
- Cell Line: A549_LUNG score (5.48111609084403E-05)
- Cell Line: GI1_CENTRAL_NERVOUS_SYSTEM score (1.45624549510359E-05)
- Cell Line: HS936T_SKIN score (9.77822651385471E-09)
- Cell Line: HS944T_SKIN score (0.0001293445518615)
- Cell Line: HSC5_SKIN score (1.53688343912269E-09)
- Cell Line: IPC298_SKIN score (0.0413289105714881)
- Experimental Setup: Timecourse-Synthetic Lethality
- GIST: A-phenotypic negative genetic interaction
- Library: Digenic Paralog CRISPR library
- Significance Threshold: GEMINI FDR < 0.05
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| UBE2A UBE2B | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| UBE2B UBE2A | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| UBE2B UBE2A | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.8911 | BioGRID | 2259894 | |
| UBE2B UBE2A | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.922 | BioGRID | 3090077 |
Curated By
- BioGRID