GSK3A
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- activation of signaling protein activity involved in unfolded protein response [TAS]
- cardiac left ventricle morphogenesis [ISS]
- cellular protein metabolic process [TAS]
- cellular response to insulin stimulus [IMP]
- cellular response to interleukin-3 [ISS]
- endoplasmic reticulum unfolded protein response [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- extrinsic apoptotic signaling pathway in absence of ligand [ISS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [ISS]
- negative regulation of TOR signaling [ISS]
- negative regulation of UDP-glucose catabolic process [IC]
- negative regulation of canonical Wnt signaling pathway [TAS]
- negative regulation of cell growth involved in cardiac muscle cell development [ISS]
- negative regulation of glucose import [IMP]
- negative regulation of glycogen (starch) synthase activity [TAS]
- negative regulation of glycogen biosynthetic process [TAS]
- negative regulation of insulin receptor signaling pathway [IMP]
- negative regulation of transferase activity [IMP]
- negative regulation of type B pancreatic cell development [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- phosphatidylinositol-mediated signaling [TAS]
- positive regulation of adrenergic receptor signaling pathway [ISS]
- positive regulation of cAMP biosynthetic process [ISS]
- positive regulation of glycogen (starch) synthase activity [ISS]
- positive regulation of heart contraction [ISS]
- positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway [ISS]
- positive regulation of protein catabolic process [NAS]
- proteasome-mediated ubiquitin-dependent protein catabolic process [ISS]
- protein phosphorylation [IDA]
- regulation of systemic arterial blood pressure [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GSK3B
Gene Ontology Biological Process
- ER overload response [IDA]
- Fc-epsilon receptor signaling pathway [TAS]
- axon guidance [TAS]
- canonical Wnt signaling pathway [IC, IDA]
- cellular response to interleukin-3 [ISS]
- circadian rhythm [ISS]
- epidermal growth factor receptor signaling pathway [TAS]
- epithelial to mesenchymal transition [IMP]
- extrinsic apoptotic signaling pathway in absence of ligand [ISS]
- fibroblast growth factor receptor signaling pathway [TAS]
- glycogen metabolic process [IDA]
- hippocampus development [IMP]
- innate immune response [TAS]
- intracellular signal transduction [IDA]
- negative regulation of NFAT protein import into nucleus [IMP]
- negative regulation of apoptotic process [IDA]
- negative regulation of canonical Wnt signaling pathway [TAS]
- negative regulation of glycogen (starch) synthase activity [TAS]
- negative regulation of glycogen biosynthetic process [TAS]
- negative regulation of protein binding [IDA]
- negative regulation of protein complex assembly [IMP]
- negative regulation of type B pancreatic cell development [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- peptidyl-serine phosphorylation [IDA]
- phosphatidylinositol-mediated signaling [TAS]
- positive regulation of Rac GTPase activity [IMP]
- positive regulation of cell-matrix adhesion [IMP]
- positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway [ISS]
- positive regulation of protein binding [ISS]
- positive regulation of protein catabolic process [IC]
- positive regulation of protein complex assembly [IDA]
- positive regulation of protein export from nucleus [IDA]
- protein phosphorylation [IDA]
- regulation of microtubule-based process [IMP]
- superior temporal gyrus development [IMP]
Gene Ontology Molecular Function- NF-kappaB binding [IPI]
- RNA polymerase II transcription factor binding [IPI]
- beta-catenin binding [IPI]
- kinase activity [IDA, TAS]
- p53 binding [IDA]
- protein binding [IPI]
- protein kinase A catalytic subunit binding [IPI]
- protein kinase binding [IPI]
- protein serine/threonine kinase activity [IDA, ISS]
- tau-protein kinase activity [IDA]
- ubiquitin protein ligase binding [IPI]
- NF-kappaB binding [IPI]
- RNA polymerase II transcription factor binding [IPI]
- beta-catenin binding [IPI]
- kinase activity [IDA, TAS]
- p53 binding [IDA]
- protein binding [IPI]
- protein kinase A catalytic subunit binding [IPI]
- protein kinase binding [IPI]
- protein serine/threonine kinase activity [IDA, ISS]
- tau-protein kinase activity [IDA]
- ubiquitin protein ligase binding [IPI]
Gene Ontology Cellular Component
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Paralog knockout profiling identifies DUSP4 and DUSP6 as a digenic dependence in MAPK pathway-driven cancers.
Although single-gene perturbation screens have revealed a number of new targets, vulnerabilities specific to frequently altered drivers have not been uncovered. An important question is whether the compensatory relationship between functionally redundant genes masks potential therapeutic targets in single-gene perturbation studies. To identify digenic dependencies, we developed a CRISPR paralog targeting library to investigate the viability effects of disrupting 3,284 ... [more]
Quantitative Score
- 0.027849587 [Confidence Score]
Throughput
- High Throughput
Additional Notes
- CRISPR GI screen
- Cell Line: PK1_PANCREAS score (0.0019936343258793)
- Cell Line: GI1_CENTRAL_NERVOUS_SYSTEM score (0.0278495872859318)
- Experimental Setup: Timecourse-Synthetic Lethality
- GIST: A-phenotypic negative genetic interaction
- Library: Digenic Paralog CRISPR library
- Significance Threshold: GEMINI FDR < 0.05
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GSK3A GSK3B | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3356947 | |
| GSK3B GSK3A | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3537656 | |
| GSK3A GSK3B | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3050097 | |
| GSK3B GSK3A | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | 3537907 |
Curated By
- BioGRID