PIP5K1A
Gene Ontology Biological Process
- actin cytoskeleton reorganization [IMP]
- activation of Rac GTPase activity [IMP]
- cell chemotaxis [IMP]
- cell migration [NAS]
- focal adhesion assembly [IMP]
- glycerophospholipid metabolic process [TAS]
- keratinocyte differentiation [TAS]
- phagocytosis [TAS]
- phosphatidylinositol biosynthetic process [TAS]
- phosphatidylinositol phosphorylation [IDA, TAS]
- phospholipid biosynthetic process [IDA]
- phospholipid metabolic process [TAS]
- protein targeting to plasma membrane [IMP]
- ruffle assembly [IMP]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PIP5K1C
Gene Ontology Biological Process
- actin cytoskeleton organization [TAS]
- adherens junction assembly [TAS]
- axon guidance [TAS]
- clathrin-mediated endocytosis [TAS]
- neutrophil chemotaxis [TAS]
- phagocytosis [TAS]
- phosphatidylinositol biosynthetic process [TAS]
- phospholipid metabolic process [TAS]
- single organismal cell-cell adhesion [TAS]
- small molecule metabolic process [TAS]
- synaptic vesicle endocytosis [TAS]
- synaptic vesicle exocytosis [TAS]
Gene Ontology Molecular Function
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Paralog knockout profiling identifies DUSP4 and DUSP6 as a digenic dependence in MAPK pathway-driven cancers.
Although single-gene perturbation screens have revealed a number of new targets, vulnerabilities specific to frequently altered drivers have not been uncovered. An important question is whether the compensatory relationship between functionally redundant genes masks potential therapeutic targets in single-gene perturbation studies. To identify digenic dependencies, we developed a CRISPR paralog targeting library to investigate the viability effects of disrupting 3,284 ... [more]
Quantitative Score
- 0.031468792 [Confidence Score]
Throughput
- High Throughput
Additional Notes
- CRISPR GI screen
- Cell Line: MELJUSO_SKIN score (0.0033427712265505)
- Cell Line: PATU8988S_PANCREAS score (0.0138441739525146)
- Cell Line: PK1_PANCREAS score (4.93144998758988E-05)
- Cell Line: GI1_CENTRAL_NERVOUS_SYSTEM score (0.0314687922139696)
- Experimental Setup: Timecourse-Synthetic Lethality
- GIST: A-phenotypic negative genetic interaction
- Library: Digenic Paralog CRISPR library
- Significance Threshold: GEMINI FDR < 0.05
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PIP5K1A PIP5K1C | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 1196832 | |
| PIP5K1A PIP5K1C | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 2220215 |
Curated By
- BioGRID