P4HB
Gene Ontology Biological Process
- cellular response to hypoxia [IMP]
- extracellular matrix organization [TAS]
- lipoprotein metabolic process [TAS]
- peptidyl-proline hydroxylation to 4-hydroxy-L-proline [IDA]
- protein folding [IBA]
- regulation of oxidative stress-induced intrinsic apoptotic signaling pathway [IMP]
- response to endoplasmic reticulum stress [IMP]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
PDIA3
Gene Ontology Biological Process
- antigen processing and presentation of exogenous peptide antigen via MHC class I [TAS]
- antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent [TAS]
- antigen processing and presentation of peptide antigen via MHC class I [TAS]
- cellular protein metabolic process [TAS]
- post-translational protein modification [TAS]
- protein N-linked glycosylation via asparagine [TAS]
- protein folding [IBA, TAS]
- protein import into nucleus [TAS]
- protein retention in ER lumen [TAS]
- proteolysis [TAS]
- response to endoplasmic reticulum stress [IBA]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Paralog knockout profiling identifies DUSP4 and DUSP6 as a digenic dependence in MAPK pathway-driven cancers.
Although single-gene perturbation screens have revealed a number of new targets, vulnerabilities specific to frequently altered drivers have not been uncovered. An important question is whether the compensatory relationship between functionally redundant genes masks potential therapeutic targets in single-gene perturbation studies. To identify digenic dependencies, we developed a CRISPR paralog targeting library to investigate the viability effects of disrupting 3,284 ... [more]
Quantitative Score
- 0.014234362 [Confidence Score]
Throughput
- High Throughput
Additional Notes
- CRISPR GI screen
- Cell Line: HSC5_SKIN score (0.0142343616533505)
- Experimental Setup: Timecourse-Synthetic Lethality
- GIST: A-phenotypic negative genetic interaction
- Library: Digenic Paralog CRISPR library
- Significance Threshold: GEMINI FDR < 0.05
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PDIA3 P4HB | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 1 | BioGRID | 1266021 |
Curated By
- BioGRID