Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Paralog knockout profiling identifies DUSP4 and DUSP6 as a digenic dependence in MAPK pathway-driven cancers.

Ito T, Young MJ, Li R, Jain S, Wernitznig A, Krill-Burger JM, Lemke CT, Monducci D, Rodriguez DJ, Chang L, Dutta S, Pal D, Paolella BR, Rothberg MV, Root DE, Johannessen CM, Parida L, Getz G, Vazquez F, Doench JG, Zamanighomi M, Sellers WR

Although single-gene perturbation screens have revealed a number of new targets, vulnerabilities specific to frequently altered drivers have not been uncovered. An important question is whether the compensatory relationship between functionally redundant genes masks potential therapeutic targets in single-gene perturbation studies. To identify digenic dependencies, we developed a CRISPR paralog targeting library to investigate the viability effects of disrupting 3,284 ... [more]

Nat Genet Dec. 01, 2021; 53(12);1664-1672 [Pubmed: 34857952]

Quantitative Score

0.019693899 [Confidence Score]

Throughput

High Throughput

Additional Notes

CRISPR GI screen
Cell Line: MEL202_UVEA score (0.0196938987468192)
Experimental Setup: Timecourse-Synthetic Lethality
GIST: A-phenotypic negative genetic interaction
Library: Digenic Paralog CRISPR library
Significance Threshold: GEMINI FDR < 0.05

Curated By

BioGRID

Interaction Summary

HECW2

Gene Ontology Biological Process

Gene Ontology Molecular Function

ubiquitin-protein transferase activity [IBA]

Gene Ontology Cellular Component

SMURF1