GFPT1
Gene Ontology Biological Process
- UDP-N-acetylglucosamine biosynthetic process [TAS]
- activation of signaling protein activity involved in unfolded protein response [TAS]
- cellular protein metabolic process [TAS]
- circadian regulation of gene expression [ISS]
- dolichol-linked oligosaccharide biosynthetic process [TAS]
- endoplasmic reticulum unfolded protein response [TAS]
- energy reserve metabolic process [TAS]
- post-translational protein modification [TAS]
- protein N-linked glycosylation via asparagine [TAS]
Gene Ontology Cellular Component
GFPT2
Gene Ontology Biological Process
- UDP-N-acetylglucosamine biosynthetic process [TAS]
- cellular protein metabolic process [TAS]
- dolichol-linked oligosaccharide biosynthetic process [TAS]
- energy reserve metabolic process [TAS]
- fructose 6-phosphate metabolic process [TAS]
- post-translational protein modification [TAS]
- protein N-linked glycosylation via asparagine [TAS]
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Paralog knockout profiling identifies DUSP4 and DUSP6 as a digenic dependence in MAPK pathway-driven cancers.
Although single-gene perturbation screens have revealed a number of new targets, vulnerabilities specific to frequently altered drivers have not been uncovered. An important question is whether the compensatory relationship between functionally redundant genes masks potential therapeutic targets in single-gene perturbation studies. To identify digenic dependencies, we developed a CRISPR paralog targeting library to investigate the viability effects of disrupting 3,284 ... [more]
Quantitative Score
- 0.013018348 [Confidence Score]
Throughput
- High Throughput
Additional Notes
- CRISPR GI screen
- Cell Line: MELJUSO_SKIN score (0.013018347811242)
- Experimental Setup: Timecourse-Synthetic Lethality
- GIST: A-phenotypic negative genetic interaction
- Library: Digenic Paralog CRISPR library
- Significance Threshold: GEMINI FDR < 0.05
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GFPT2 GFPT1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 2223521 | |
| GFPT2 GFPT1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9999 | BioGRID | 3095704 |
Curated By
- BioGRID