An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Phosphorylated tau interactome in the human Alzheimer's disease brain.

Drummond E, Pires G, MacMurray C, Askenazi M, Nayak S, Bourdon M, Safar J, Ueberheide B, Wisniewski T

Accumulation of phosphorylated tau is a key pathological feature of Alzheimer's disease. Phosphorylated tau accumulation causes synaptic impairment, neuronal dysfunction and formation of neurofibrillary tangles. The pathological actions of phosphorylated tau are mediated by surrounding neuronal proteins; however, a comprehensive understanding of the proteins that phosphorylated tau interacts with in Alzheimer's disease is surprisingly limited. Therefore, the aim of this ... [more]

Brain Sep. 01, 2020; 143(9);2803-2817 [Pubmed: 32812023]

Throughput

High Throughput

Additional Notes

High confidence interactors of phosphorylated Tau (pTau) were identified as those with a SAINT score > 0.65, which corresponded to a Bayesian false discovery rate of 10%.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MAPT GPI	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Tracy TE (2022)	High	-	BioGRID	3662156

Curated By

BioGRID

Interaction Summary

MAPT

Gene Ontology Biological Process

Gene Ontology Molecular Function

SH3 domain binding [IPI]apolipoprotein binding [IPI]enzyme binding [IPI]lipoprotein particle binding [IPI]microtubule binding [IDA]protein binding [IPI]structural constituent of cytoskeleton [TAS]

Gene Ontology Cellular Component

GPI