CFTR
Gene Ontology Biological Process
- cellular response to cAMP [ISS]
- chloride transmembrane transport [IDA, ISS, NAS, TAS]
- intracellular pH elevation [ISS]
- membrane hyperpolarization [ISS]
- positive regulation of voltage-gated chloride channel activity [IDA]
- respiratory gaseous exchange [TAS]
- sperm capacitation [ISS]
- transmembrane transport [TAS]
- transport [TAS]
Gene Ontology Molecular Function- ATP-binding and phosphorylation-dependent chloride channel activity [TAS]
- PDZ domain binding [IDA]
- bicarbonate transmembrane transporter activity [ISS]
- channel-conductance-controlling ATPase activity [NAS]
- chloride channel activity [IDA]
- chloride channel inhibitor activity [IDA]
- chloride transmembrane transporter activity [ISS]
- enzyme binding [IPI]
- protein binding [IPI]
- ATP-binding and phosphorylation-dependent chloride channel activity [TAS]
- PDZ domain binding [IDA]
- bicarbonate transmembrane transporter activity [ISS]
- channel-conductance-controlling ATPase activity [NAS]
- chloride channel activity [IDA]
- chloride channel inhibitor activity [IDA]
- chloride transmembrane transporter activity [ISS]
- enzyme binding [IPI]
- protein binding [IPI]
Gene Ontology Cellular Component
ADCYAP1
Gene Ontology Biological Process
- activation of adenylate cyclase activity [TAS]
- cAMP-mediated signaling [IMP]
- cell-cell signaling [TAS]
- female pregnancy [TAS]
- neuron projection development [IDA]
- neuropeptide signaling pathway [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- positive regulation of ERK1 and ERK2 cascade [IDA]
- positive regulation of Rap GTPase activity [IDA]
- positive regulation of adenylate cyclase activity involved in G-protein coupled receptor signaling pathway [IDA]
- positive regulation of protein kinase activity [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of G-protein coupled receptor protein signaling pathway [IDA]
- regulation of protein localization [IDA]
- transmembrane receptor protein tyrosine kinase signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PCA
A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.
Publication
CFTR interactome mapping using the mammalian membrane two-hybrid high-throughput screening system.
Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) is a chloride and bicarbonate channel in secretory epithelia with a critical role in maintaining fluid homeostasis. Mutations in CFTR are associated with Cystic Fibrosis (CF), the most common lethal autosomal recessive disorder in Caucasians. While remarkable treatment advances have been made recently in the form of modulator drugs directly rescuing CFTR dysfunction, there ... [more]
Throughput
- High Throughput
Additional Notes
- Mammalian-membrane two-hybrid assay (MaMTH)
- Split ubiquitin-based method
- Wild type CFTR
Curated By
- BioGRID