CFTR
Gene Ontology Biological Process
- cellular response to cAMP [ISS]
- chloride transmembrane transport [IDA, ISS, NAS, TAS]
- intracellular pH elevation [ISS]
- membrane hyperpolarization [ISS]
- positive regulation of voltage-gated chloride channel activity [IDA]
- respiratory gaseous exchange [TAS]
- sperm capacitation [ISS]
- transmembrane transport [TAS]
- transport [TAS]
Gene Ontology Molecular Function- ATP-binding and phosphorylation-dependent chloride channel activity [TAS]
- PDZ domain binding [IDA]
- bicarbonate transmembrane transporter activity [ISS]
- channel-conductance-controlling ATPase activity [NAS]
- chloride channel activity [IDA]
- chloride channel inhibitor activity [IDA]
- chloride transmembrane transporter activity [ISS]
- enzyme binding [IPI]
- protein binding [IPI]
- ATP-binding and phosphorylation-dependent chloride channel activity [TAS]
- PDZ domain binding [IDA]
- bicarbonate transmembrane transporter activity [ISS]
- channel-conductance-controlling ATPase activity [NAS]
- chloride channel activity [IDA]
- chloride channel inhibitor activity [IDA]
- chloride transmembrane transporter activity [ISS]
- enzyme binding [IPI]
- protein binding [IPI]
Gene Ontology Cellular Component
VAPB
Gene Ontology Biological Process
- COPII-coated vesicle budding [IMP]
- ER to Golgi vesicle-mediated transport [IMP]
- activation of signaling protein activity involved in unfolded protein response [IDA]
- cellular calcium ion homeostasis [IMP]
- endoplasmic reticulum organization [IMP]
- endoplasmic reticulum unfolded protein response [IMP]
- modulation by virus of host morphology or physiology [IDA]
- negative regulation by host of viral genome replication [IDA]
- negative regulation by host of viral release from host cell [IDA]
- negative regulation by virus of viral protein levels in host cell [IDA]
- positive regulation by host of viral genome replication [IDA]
- positive regulation by host of viral release from host cell [IDA]
- positive regulation of viral genome replication [IMP]
- small molecule metabolic process [TAS]
- sphingolipid biosynthetic process [TAS]
- sphingolipid metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PCA
A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.
Publication
CFTR interactome mapping using the mammalian membrane two-hybrid high-throughput screening system.
Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) is a chloride and bicarbonate channel in secretory epithelia with a critical role in maintaining fluid homeostasis. Mutations in CFTR are associated with Cystic Fibrosis (CF), the most common lethal autosomal recessive disorder in Caucasians. While remarkable treatment advances have been made recently in the form of modulator drugs directly rescuing CFTR dysfunction, there ... [more]
Throughput
- High Throughput
Additional Notes
- Mammalian-membrane two-hybrid assay (MaMTH)
- Split ubiquitin-based method
- Wild type CFTR
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CFTR VAPB | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 2448185 |
Curated By
- BioGRID